MRS Agar

Recommended medium for the growth of lactobacilli. Suspend 62 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 12 minutes. Cool to 45-50ºC, mix well and dispense into plates.

• Dextrose 20
  
• Bacteriological Peptone 10
  
• Beef Extract 8
  
• Sodium Acetate 5
  
• Yeast Extract 4
  
• Dipotassium Phosphate 2
  
• Ammonium Citrate 2
  
• Tween80 1
  
• Magnesium Sulfate 0.2
  
• Manganase Sulfate 0.05
  
• Bacteriological Agar 10

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